Conversion of free 3β-hydroxy-5-ene-steroids by incubation with Helix pomatia

The incubation of the 3β-hydroxy-5-ene-steroids, pregnenolone, 5-pregnene-3β,20a-diol and 17-hydroxypregnenolone with an enzyme preparation from Helix pomatia used to hydrolyse steroid conjugates, may lead to changed structures. Using capillary gas chromatography the corresponding 4-ene-steroids wer...

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Veröffentlicht in:	Journal of steroid biochemistry 1982-05, Vol.16 (5), p.701-703
Hauptverfasser:	Vanluchene, E., Eechaute, W., Vandekerckhove, D.
Format:	Artikel
Sprache:	eng
Schlagworte:	17-alpha-Hydroxypregnenolone - metabolism Animals Chromatography, Gas Helix (Snails) - metabolism Hydroxycorticosteroids - metabolism Pregnanolone - metabolism Pregnenolone - analogs & derivatives Pregnenolone - metabolism
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Beschreibung
Zusammenfassung:	The incubation of the 3β-hydroxy-5-ene-steroids, pregnenolone, 5-pregnene-3β,20a-diol and 17-hydroxypregnenolone with an enzyme preparation from Helix pomatia used to hydrolyse steroid conjugates, may lead to changed structures. Using capillary gas chromatography the corresponding 4-ene-steroids were identified. Therefore, when using Helix pomatia extract the measurement of both 3β-hydroxy-5-ene-and 4-ene-3-oxo-steroids in biological fluids may be unreliable.
ISSN:	0022-4731
DOI:	10.1016/0022-4731(82)90107-8