Distribution of acetylated forms of nucleosomal histones in fractionated chromatin

Distribution of acetylated forms of nucleosomal histones in fractionated chromatin

Hyperacetylated chromatin was isolated from Ehrlich ascites tumor cells grown in n-butyrate containing medium and fractionated by mild digestion with deoxyribonuclease II and precipitation with MgCl 2. The most highly acetylated forms of histones H4 and H3 as well as the acetylated subspecies of H2B...

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Veröffentlicht in:Archives of biochemistry and biophysics 1982-06, Vol.216 (1), p.88-92
Hauptverfasser: Georgieva, Elena I., Pashev, Iliya G., Tsanev, Roumen G.
Format: Artikel
Sprache:eng
Schlagworte:
Acetylation
Animals
Carcinoma, Ehrlich Tumor - analysis
Chromatin - analysis
Electrophoresis, Polyacrylamide Gel
Histones - analysis
Kinetics
Mice
Molecular Weight
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Zusammenfassung:Hyperacetylated chromatin was isolated from Ehrlich ascites tumor cells grown in n-butyrate containing medium and fractionated by mild digestion with deoxyribonuclease II and precipitation with MgCl 2. The most highly acetylated forms of histones H4 and H3 as well as the acetylated subspecies of H2B were found almost entirely associated with the putatively active Mg 2+-soluble chromatin fraction. The Mg 2+-insoluble fraction contained mainly mono- and diacetylated molecules of H4 and H3.
ISSN:0003-9861
1096-0384
DOI:10.1016/0003-9861(82)90191-6