Inhibitory Effects of Melatonin, Prostaglandin E1, Cyclic Amp, Dibutyrylcyclic AMP and Theophylline on Rat Seminiferous Tubular Contractility in vitro
Autorhythmic contractions (frequency) of seminiferous tubules from adult male rats responded biphasically to PGE 1 in vitro . At low concentrations (10 -9 M), the frequency increased, but at higher concentrations (10 -7 or 10 -8 M), the rate of contractions was diminished. Depth or intensity of tubu...
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Veröffentlicht in: | Biology of reproduction 1978-08, Vol.19 (1), p.217-222 |
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Sprache: | eng |
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Zusammenfassung: | Autorhythmic contractions (frequency) of seminiferous tubules from adult male rats responded
biphasically to PGE 1 in vitro . At low concentrations (10 -9 M), the frequency increased, but at
higher concentrations (10 -7 or 10 -8 M), the rate of contractions was diminished. Depth or intensity
of tubular contractions showed a similar trend with a decreased magnitude at 10 -6 M. Tonus of the
tubules was increased at 10 -9 M concentration of PGE 1 , but not at higher concentrations. Silastic
implants of either cyclic AMP or dibutyryl cyclic AMP decreased contractility of rat seminiferous
tubules in vitro as did the incubation of seminiferous tubules with dibutyryl cyclic AMP (2 mM)
and theophylline (2.8 x 10 -4 M). Subcutaneous injections of melatonin (250, 500 and 1,000µ
g/day) also diminished both the frequency and depth of contractions, but not tonus of
siminiferous tubules. It was concluded that cyclic AMP could be a second messenger for PGE 1 , but
not for melatonin. |
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ISSN: | 0006-3363 1529-7268 |
DOI: | 10.1095/biolreprod19.1.217 |