Identification and determination of thyroxine using gas-liquid chromatography
A quantitative gas-liquid chromatographic assay method has been developed for the thyroid hormone thyroxine as a bulk substance. The trimethylsilylated derivative is chromatographed with an internal standard on an OV-101 column by means of flame ionization detection. Analogs of thyroxine can be iden...
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| Veröffentlicht in: | Analytical biochemistry 1978-07, Vol.87 (2), p.496-505 |
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| Hauptverfasser: | , , |
| Format: | Artikel |
| Sprache: | eng |
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| Online-Zugang: | Volltext |
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| Zusammenfassung: | A quantitative gas-liquid chromatographic assay method has been developed for the thyroid hormone thyroxine as a bulk substance. The trimethylsilylated derivative is chromatographed with an internal standard on an OV-101 column by means of flame ionization detection. Analogs of thyroxine can be identified and quantitated as possible contaminants. Electron-capture detection of thyroxine at the picogram level has been demonstrated. Complete silylation has been confirmed. |
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| ISSN: | 0003-2697 1096-0309 |
| DOI: | 10.1016/0003-2697(78)90699-1 |