Subcultivation of human hair follicle keratinocytes
A method is described for the subcultivation of human hair follicle keratinocytes. Primary cultures of these cells were grown on bovine eye lens capsules. Fragments of the colonies could successfully be transplanted onto new capsules. After two subcultivation steps, the keratinocytes remain diploid...
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| Veröffentlicht in: | Experimental cell research 1982-06, Vol.139 (2), p.439-443 |
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| container_title | Experimental cell research |
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| creator | Weterings, P.J.J.M. Vermorken, A.J.M. Bloemendal, H. |
| description | A method is described for the subcultivation of human hair follicle keratinocytes. Primary cultures of these cells were grown on bovine eye lens capsules. Fragments of the colonies could successfully be transplanted onto new capsules. After two subcultivation steps, the keratinocytes remain diploid and still exhibit the same pattern of protein biosynthesis as primary cultures. The cultured hair follicle cells may be useful in investigations on genetically determined sensitivity towards carcinogens. |
| doi_str_mv | 10.1016/0014-4827(82)90275-0 |
| format | Article |
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Primary cultures of these cells were grown on bovine eye lens capsules. Fragments of the colonies could successfully be transplanted onto new capsules. After two subcultivation steps, the keratinocytes remain diploid and still exhibit the same pattern of protein biosynthesis as primary cultures. The cultured hair follicle cells may be useful in investigations on genetically determined sensitivity towards carcinogens.</description><identifier>ISSN: 0014-4827</identifier><identifier>EISSN: 1090-2422</identifier><identifier>DOI: 10.1016/0014-4827(82)90275-0</identifier><identifier>PMID: 7084328</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Animals ; Cattle ; Cells, Cultured ; DNA - analysis ; Electrophoresis, Polyacrylamide Gel ; Epidermal Cells ; Flow Cytometry ; Hair - cytology ; Humans ; Lens, Crystalline ; Protein Biosynthesis</subject><ispartof>Experimental cell research, 1982-06, Vol.139 (2), p.439-443</ispartof><rights>1982</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c272t-66970488eaa6b540e01c167a6c070017e587c896360f6b65be5b444dcb975a823</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0014-4827(82)90275-0$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7084328$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Weterings, P.J.J.M.</creatorcontrib><creatorcontrib>Vermorken, A.J.M.</creatorcontrib><creatorcontrib>Bloemendal, H.</creatorcontrib><title>Subcultivation of human hair follicle keratinocytes</title><title>Experimental cell research</title><addtitle>Exp Cell Res</addtitle><description>A method is described for the subcultivation of human hair follicle keratinocytes. Primary cultures of these cells were grown on bovine eye lens capsules. Fragments of the colonies could successfully be transplanted onto new capsules. After two subcultivation steps, the keratinocytes remain diploid and still exhibit the same pattern of protein biosynthesis as primary cultures. The cultured hair follicle cells may be useful in investigations on genetically determined sensitivity towards carcinogens.</description><subject>Animals</subject><subject>Cattle</subject><subject>Cells, Cultured</subject><subject>DNA - analysis</subject><subject>Electrophoresis, Polyacrylamide Gel</subject><subject>Epidermal Cells</subject><subject>Flow Cytometry</subject><subject>Hair - cytology</subject><subject>Humans</subject><subject>Lens, Crystalline</subject><subject>Protein Biosynthesis</subject><issn>0014-4827</issn><issn>1090-2422</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1982</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kD1PwzAQhi0EKqXwD0DKhGAInB3HdhYkVPElVWIAZstxLqohicFOKvXfk9KqI9MN78fdPYScU7ihQMUtAOUpV0xeKXZdAJN5CgdkSqGAlHHGDsl0bzkmJzF-AoBSVEzIRILiGVNTkr0NpR2a3q1M73yX-DpZDq3pkqVxIal90zjbYPKFYdQ7b9c9xlNyVJsm4tluzsjH48P7_DldvD69zO8XqWWS9akQhQSuFBojypwDArVUSCMsyPEwibmSVhUiE1CLUuQl5iXnvLJlIXOjWDYjl9ve7-B_Boy9bl202DSmQz9ELTmMG4p8NPKt0QYfY8BafwfXmrDWFPSGld6A0BsQWjH9x0rDGLvY9Q9li9U-tIMz6ndbHccnVw6DjtZhZ7FyAW2vK-_-X_ALA_Z22w</recordid><startdate>198206</startdate><enddate>198206</enddate><creator>Weterings, P.J.J.M.</creator><creator>Vermorken, A.J.M.</creator><creator>Bloemendal, H.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>198206</creationdate><title>Subcultivation of human hair follicle keratinocytes</title><author>Weterings, P.J.J.M. ; Vermorken, A.J.M. ; Bloemendal, H.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c272t-66970488eaa6b540e01c167a6c070017e587c896360f6b65be5b444dcb975a823</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1982</creationdate><topic>Animals</topic><topic>Cattle</topic><topic>Cells, Cultured</topic><topic>DNA - analysis</topic><topic>Electrophoresis, Polyacrylamide Gel</topic><topic>Epidermal Cells</topic><topic>Flow Cytometry</topic><topic>Hair - cytology</topic><topic>Humans</topic><topic>Lens, Crystalline</topic><topic>Protein Biosynthesis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Weterings, P.J.J.M.</creatorcontrib><creatorcontrib>Vermorken, A.J.M.</creatorcontrib><creatorcontrib>Bloemendal, H.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Experimental cell research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Weterings, P.J.J.M.</au><au>Vermorken, A.J.M.</au><au>Bloemendal, H.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Subcultivation of human hair follicle keratinocytes</atitle><jtitle>Experimental cell research</jtitle><addtitle>Exp Cell Res</addtitle><date>1982-06</date><risdate>1982</risdate><volume>139</volume><issue>2</issue><spage>439</spage><epage>443</epage><pages>439-443</pages><issn>0014-4827</issn><eissn>1090-2422</eissn><abstract>A method is described for the subcultivation of human hair follicle keratinocytes. 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| fulltext | fulltext |
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| ispartof | Experimental cell research, 1982-06, Vol.139 (2), p.439-443 |
| issn | 0014-4827 1090-2422 |
| language | eng |
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| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Animals Cattle Cells, Cultured DNA - analysis Electrophoresis, Polyacrylamide Gel Epidermal Cells Flow Cytometry Hair - cytology Humans Lens, Crystalline Protein Biosynthesis |
| title | Subcultivation of human hair follicle keratinocytes |
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