Subcultivation of human hair follicle keratinocytes

Subcultivation of human hair follicle keratinocytes

A method is described for the subcultivation of human hair follicle keratinocytes. Primary cultures of these cells were grown on bovine eye lens capsules. Fragments of the colonies could successfully be transplanted onto new capsules. After two subcultivation steps, the keratinocytes remain diploid...

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Veröffentlicht in:Experimental cell research 1982-06, Vol.139 (2), p.439-443
Hauptverfasser: Weterings, P.J.J.M., Vermorken, A.J.M., Bloemendal, H.
Format: Artikel
Sprache:eng
Schlagworte:
Animals
Cattle
Cells, Cultured
DNA - analysis
Electrophoresis, Polyacrylamide Gel
Epidermal Cells
Flow Cytometry
Hair - cytology
Humans
Lens, Crystalline
Protein Biosynthesis
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Zusammenfassung:A method is described for the subcultivation of human hair follicle keratinocytes. Primary cultures of these cells were grown on bovine eye lens capsules. Fragments of the colonies could successfully be transplanted onto new capsules. After two subcultivation steps, the keratinocytes remain diploid and still exhibit the same pattern of protein biosynthesis as primary cultures. The cultured hair follicle cells may be useful in investigations on genetically determined sensitivity towards carcinogens.
ISSN:0014-4827
1090-2422
DOI:10.1016/0014-4827(82)90275-0