A Short Review of Techniques for the Localisation of γ-Glutamyl Transferase Isoenzymes after Electrophoresis

A Short Review of Techniques for the Localisation of γ-Glutamyl Transferase Isoenzymes after Electrophoresis

Summary The development of a sensitive method for the localisation of γ-glutamyl transferase isoenzymes after electrophoresis on various media is described. Recommendations on final conditions are given for optimal concentrations of substrate, acceptor and buffer, and for pH of the reaction mixture....

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Veröffentlicht in:Annals of clinical biochemistry 1978-01, Vol.15 (1-6), p.146-150
Hauptverfasser: Wenham, P. R., Price, C. P., Sammons, H. G.
Format: Artikel
Sprache:eng
Schlagworte:
Electrophoresis
gamma-Glutamyltransferase - blood
Isoenzymes - blood
Methods
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Zusammenfassung:Summary The development of a sensitive method for the localisation of γ-glutamyl transferase isoenzymes after electrophoresis on various media is described. Recommendations on final conditions are given for optimal concentrations of substrate, acceptor and buffer, and for pH of the reaction mixture. The reaction conditions are compared with other isoenzyme localisation techniques previously reported.
ISSN:0004-5632
1758-1001
DOI:10.1177/000456327801500130