The initiation of TMV infection in isolated protoplasts by polyethylene glycol

The initiation of TMV infection in isolated protoplasts by polyethylene glycol

Tobacco mosaic virus (TMV) particles bind to isolated tomato protoplasts and following treatment with polyethylene glycol (PEG) initiate infection. Studies indicate that the procedure involves two steps, TMV binding to the plasmalemma, followed by PEG-initiated infection. The percentage of protoplas...

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Veröffentlicht in:Virology (New York, N.Y.) N.Y.), 1978-06, Vol.87 (2), p.459-462
Hauptverfasser: Cassells, Alan C., Barlass, Martin
Format: Artikel
Sprache:eng
Schlagworte:
Adsorption
Cell Membrane - drug effects
Cell Membrane - microbiology
Plants - drug effects
Plants - microbiology
Polyethylene Glycols - pharmacology
Protoplasts - microbiology
Tobacco Mosaic Virus - growth & development
Virus Replication
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Zusammenfassung:Tobacco mosaic virus (TMV) particles bind to isolated tomato protoplasts and following treatment with polyethylene glycol (PEG) initiate infection. Studies indicate that the procedure involves two steps, TMV binding to the plasmalemma, followed by PEG-initiated infection. The percentage of protoplasts infected is routinely ca. 60% and mean virus production per infected protoplast is ca. 1.5 × 10 6 TMV particles. It is suggested that PEG may induce TMV/plasmalemma fusion.
ISSN:0042-6822
1096-0341
DOI:10.1016/0042-6822(78)90147-2