A rapid assay for dipeptidyl aminopeptidase III in human erythrocytes

A simple, rapid procedure for the assay of dipeptidyl aminopeptidase III in human erythrocytes has been developed. The cells are lysed with Triton X-100, centrifuged, and the supernatant fractionated on small columns of carboxymethyl Bio-Gel A at pH 6.5. The enzyme is eluted in the void volume and c...

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Veröffentlicht in:	Analytical biochemistry 1982-01, Vol.119 (2), p.418-423
Hauptverfasser:	Jones, Theodore H.D., Kapralou, Alexia
Format:	Artikel
Sprache:	eng
Schlagworte:	Blood Proteins - analysis Chromatography, Gel - methods Chromatography, Ion Exchange - methods Dipeptidyl-Peptidases and Tripeptidyl-Peptidases - blood Endopeptidases - blood Erythrocytes - enzymology Hemoglobinometry - methods Humans In Vitro Techniques Octoxynol Polyethylene Glycols
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Beschreibung
Zusammenfassung:	A simple, rapid procedure for the assay of dipeptidyl aminopeptidase III in human erythrocytes has been developed. The cells are lysed with Triton X-100, centrifuged, and the supernatant fractionated on small columns of carboxymethyl Bio-Gel A at pH 6.5. The enzyme is eluted in the void volume and can be obtained completely separated from hemoglobin; it is then assayed by hydrolysis of Arg-Arg-β-naphthylamide. The procedure is suitable for the routine screening of large numbers of human blood samples for their DAP III activity.
ISSN:	0003-2697 1096-0309
DOI:	10.1016/0003-2697(82)90607-8