Determination of pyrimidine dimer unwinding angle by measurement of DNA electrophoretic mobility

The unwinding angle produced by the formation of one pyrimidine dimer has been estimated to be −14.3 ° ±0.2 °. This value has been obtained by titrating the number of pyrimidine dimers necessary to reduce the number of superhelical turns by one in each topoisomer obtained by treatment of a supercoil...

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Veröffentlicht in:	Journal of molecular biology 1982-02, Vol.155 (2), p.177-183
Hauptverfasser:	Ciarrocchi, Giovanni, Pedrini, Antonia M.
Format:	Artikel
Sprache:	eng
Schlagworte:	DNA DNA Topoisomerases, Type I DNA, Superhelical Escherichia coli - genetics Nucleic Acid Conformation Plasmids Pyrimidine Dimers
Online-Zugang:	Volltext
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Zusammenfassung:	The unwinding angle produced by the formation of one pyrimidine dimer has been estimated to be −14.3 ° ±0.2 °. This value has been obtained by titrating the number of pyrimidine dimers necessary to reduce the number of superhelical turns by one in each topoisomer obtained by treatment of a supercoiled DNA with DNA topoisomerase I.
ISSN:	0022-2836 1089-8638
DOI:	10.1016/0022-2836(82)90445-4