Nonspecific esterase activity in plastic-embedded tissues using Meldola Blue

Nonspecific esterase activity in plastic-embedded tissues using Meldola Blue

A method for the demonstration of nonspecific esterase activity in plastic-embedded tissues using Meldola Blue is described. Although Meldola Blue does not function as an electron carrier in this method, it may account for the short incubation time and excellent localization of the reaction product....

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Veröffentlicht in:American journal of clinical pathology 1982-04, Vol.77 (4), p.465-467
Hauptverfasser: Cannon, M S, Cannon, A M, Gelderd, J B, Bowers, D E
Format: Artikel
Sprache:eng
Schlagworte:
Carboxylesterase
Carboxylic Ester Hydrolases - analysis
Coloring Agents
Fixatives
Freezing
Histocytochemistry
Humans
Liver - enzymology
Oxazines
Paraffin
Plastics
Time Factors
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Zusammenfassung:A method for the demonstration of nonspecific esterase activity in plastic-embedded tissues using Meldola Blue is described. Although Meldola Blue does not function as an electron carrier in this method, it may account for the short incubation time and excellent localization of the reaction product. Use of Meldola Blue is an advancement in the demonstration of enzyme activity in plastic sections.
ISSN:0002-9173
1943-7722
DOI:10.1093/ajcp/77.4.465