Three-dimensional structure at 3.2 Å resolution of the complex of cytosolic aspartate aminotransferase from chicken heart with 2-oxoglutarate

Aspartate aminotransferase (EC 2.6.1.1; L-aspartate: 2-oxoglutarate aminotransferase, AATase) is one of the key enzymes of nitrogen metabolism. The tissues of vertebrates contain 2 homologous isozymic forms of this enzyme: a cytosolic and mitochondrial form. Both isoenzymes are dimeric proteins, con...

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Veröffentlicht in:	FEBS letters 1982-02, Vol.138 (1), p.113-116
Hauptverfasser:	Harutyunyan, E.G., Malashkevich, V.N., Tersyan, S.S., Kochkina, V.M., Torchinsky, Yu.M., Braunstein, A.E.
Format:	Artikel
Sprache:	eng
Schlagworte:	2-oxoglutaric acid Animals aspartate aminotransferase Aspartate Aminotransferases - metabolism cAATase, cytosolic aspartate aminotransferase Chickens cytosol DTNB, 5,5'-dithiobis-(2-nitrobenzoate) heart Ketoglutaric Acids - metabolism mAATase, mitochondrial aspartate aminotransferase MMA, methylmercuriacetate Models, Molecular Models, Structural Myocardium - enzymology p-CMB, p-chloromercuribenzoate PLP, pyridoxal-5′-phosphate X-ray diffraction
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Zusammenfassung:	Aspartate aminotransferase (EC 2.6.1.1; L-aspartate: 2-oxoglutarate aminotransferase, AATase) is one of the key enzymes of nitrogen metabolism. The tissues of vertebrates contain 2 homologous isozymic forms of this enzyme: a cytosolic and mitochondrial form. Both isoenzymes are dimeric proteins, consisting of 2 identical subunits of M sub(r) similar to 45,000. Currently, X-ray structural investigations are underway of chicken cAATase and mAATase and of porcine cAATase. Here, the authors report studies of the three-dimensional structure of chicken cAATase at 3.2 angstrom resolution.
ISSN:	0014-5793 1873-3468
DOI:	10.1016/0014-5793(82)80407-9