A rapid radioimmunoassay for human α-fetoprotein

A rapid radioimmunoassay for human α-fetoprotein

Our previous double antibody radioimmunoassay for human AFP was run at 4°C and required 48 h for completion. A method is described which permits the assay to be performed within 5 h due to an increased incubation temperature and the use of polyethylene glycol to precipitate the antigen-antibody comp...

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Veröffentlicht in:Clinica chimica acta 1978-05, Vol.86 (1), p.67-72
Hauptverfasser: Blank-Liss, W.E., Lai, P.C.W., Hay, D.M., Lorscheider, F.L.
Format: Artikel
Sprache:eng
Schlagworte:
alpha-Fetoproteins - analysis
Animals
Goats - immunology
Humans
Methods
Polyethylene Glycols
Rabbits - immunology
Radioimmunoassay - methods
Temperature
Time Factors
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Zusammenfassung:Our previous double antibody radioimmunoassay for human AFP was run at 4°C and required 48 h for completion. A method is described which permits the assay to be performed within 5 h due to an increased incubation temperature and the use of polyethylene glycol to precipitate the antigen-antibody complex. The modified radioimmunoassay was standardized against an international reference preparation of human AFP. Characteristics of this assay are discussed.
ISSN:0009-8981
1873-3492
DOI:10.1016/0009-8981(78)90459-X