An improved technique for the isolation of lymphocytes from small volumes of peripheral mouse blood

An improved technique for the isolation of lymphocytes from small volumes of peripheral mouse blood

Centrifugation of mouse blood on density gradients of polyvinylpyrrolidone-coated colloidal silica (Percoll) resulted in the simultaneous separation of platelets, lymphocytes, granulocytes and erythrocytes. By this method, we have been successful in isolating a fraction of highly purified and viable...

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Veröffentlicht in:Journal of immunological methods 1982-01, Vol.48 (3), p.269-279
Hauptverfasser: Mizobe, Fumio, Martial, Eleonora, Colby-Germinario, Susan, Livett, Bruce G.
Format: Artikel
Sprache:eng
Schlagworte:
Animals
Blood Platelets
Cell Separation - methods
Cell Survival
Centrifugation, Density Gradient
Erythrocytes
Female
Granulocytes
isopycnic centrifugation
Lymphocyte Activation
lymphocyte isolation
Lymphocytes
Male
Mice
Mice, Inbred C57BL
mouse and human blood
Percoll fractionation
Phytohemagglutinins - pharmacology
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Zusammenfassung:Centrifugation of mouse blood on density gradients of polyvinylpyrrolidone-coated colloidal silica (Percoll) resulted in the simultaneous separation of platelets, lymphocytes, granulocytes and erythrocytes. By this method, we have been successful in isolating a fraction of highly purified and viable lymphocytes from small volumes of peripheral mouse blood with good recovery.
ISSN:0022-1759
1872-7905
DOI:10.1016/0022-1759(82)90327-1