The Properties of Two Sea-Squirt Antigens
The accuracy of radioimmunoassay (RIA) has been much improved for sea-squirt antigen Gi-x, which is considered to be of higher molecular weight than antigen Ei-M, by using radiolabeled Gi-x as a tracer. Gel chromatography monitored by the improved RIA revealed a wide distribution of molecular weight...
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| Veröffentlicht in: | Journal of biochemistry (Tokyo) 1982-01, Vol.91 (1), p.247-255 |
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| container_title | Journal of biochemistry (Tokyo) |
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| creator | OKA, Satoru SUZUKI, Hidenori HASHIMOTO, Hideo JYO, Toshihiko |
| description | The accuracy of radioimmunoassay (RIA) has been much improved for sea-squirt antigen Gi-x, which is considered to be of higher molecular weight than antigen Ei-M, by using radiolabeled Gi-x as a tracer. Gel chromatography monitored by the improved RIA revealed a wide distribution of molecular weight of Gi-x type antigens, in contrast to Ei-M. However, since a considerable portion of the anti-genic activity gave a single peak in gel chromatography with Sepharose 6B, the substance in the peak fractions was isolated as a fairly homogeneous preparation and referred to as Gi-rep. Gi-rep showed distinct characteristics of Gi-x type antigens and was clearly discriminated from Ei-M by radioimmunometry in vitro. The in vitro observation also suggested that Gi-rep and Ei-M carried a common antigenic determinant (type α), but that Ei-M also carried a specific determinant (type β). The weight-average molecular weights were 1.1×105 for Gi-rep and 2.3×104 for Ei-M. Both preparations consisted of acidic glycoproteins with considerable amounts of sulfate and phosphate. |
| doi_str_mv | 10.1093/oxfordjournals.jbchem.a133681 |
| format | Article |
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Gel chromatography monitored by the improved RIA revealed a wide distribution of molecular weight of Gi-x type antigens, in contrast to Ei-M. However, since a considerable portion of the anti-genic activity gave a single peak in gel chromatography with Sepharose 6B, the substance in the peak fractions was isolated as a fairly homogeneous preparation and referred to as Gi-rep. Gi-rep showed distinct characteristics of Gi-x type antigens and was clearly discriminated from Ei-M by radioimmunometry in vitro. The in vitro observation also suggested that Gi-rep and Ei-M carried a common antigenic determinant (type α), but that Ei-M also carried a specific determinant (type β). The weight-average molecular weights were 1.1×105 for Gi-rep and 2.3×104 for Ei-M. 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Both preparations consisted of acidic glycoproteins with considerable amounts of sulfate and phosphate.</description><subject>Animals</subject><subject>Antigens - analysis</subject><subject>Antigens - immunology</subject><subject>Antigens - isolation & purification</subject><subject>Body Fluids - immunology</subject><subject>Chromatography, Gel</subject><subject>Epitopes - immunology</subject><subject>Glycoproteins - analysis</subject><subject>Molecular Weight</subject><subject>Radioimmunoassay</subject><subject>Urochordata - immunology</subject><issn>0021-924X</issn><issn>1756-2651</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1982</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVkF1LwzAYhYMoc05_gtAbBS8689EkzYUX2_yYMFBZhbGbkLap61ybLWlx_ns7WgZevRzOOe-BB4AbBIcICnJv9pmx6drUtlQbN1zHyUoXQ4UIYSE6AX3EKfMxo-gU9CHEyBc4WJyDC-fWB4kJ6YEeO6Qw7oO7aKW9d2u22la5dp7JvOjHeHOt_Pmuzm3ljcoq_9KluwRnWTOor7o7AJ_PT9Fk6s_eXl4no5mfEI4qP8EkUIorplSKlaIY6jjjRJAgVCLAPI4FgxCmKQsFpTjUhFBBGUKIpygNBRmA2_bv1ppdrV0li9wlerNRpTa1kzyACGKGm-BDG0yscc7qTG5tXij7KxGUB1TyPyrZopIdqqZ_3Q3VcaHTY7tj0_h-6-eu0vujrey3ZJxwKqeLpZyPI_pIl1B-kD9k9nof</recordid><startdate>198201</startdate><enddate>198201</enddate><creator>OKA, Satoru</creator><creator>SUZUKI, Hidenori</creator><creator>HASHIMOTO, Hideo</creator><creator>JYO, Toshihiko</creator><general>Oxford University Press</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>198201</creationdate><title>The Properties of Two Sea-Squirt Antigens</title><author>OKA, Satoru ; SUZUKI, Hidenori ; HASHIMOTO, Hideo ; JYO, Toshihiko</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c371t-c234aa7a6aad2aa520ebf739348a9427bb96000dd6895528e3359561117d1d893</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1982</creationdate><topic>Animals</topic><topic>Antigens - analysis</topic><topic>Antigens - immunology</topic><topic>Antigens - isolation & purification</topic><topic>Body Fluids - immunology</topic><topic>Chromatography, Gel</topic><topic>Epitopes - immunology</topic><topic>Glycoproteins - analysis</topic><topic>Molecular Weight</topic><topic>Radioimmunoassay</topic><topic>Urochordata - immunology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>OKA, Satoru</creatorcontrib><creatorcontrib>SUZUKI, Hidenori</creatorcontrib><creatorcontrib>HASHIMOTO, Hideo</creatorcontrib><creatorcontrib>JYO, Toshihiko</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of biochemistry (Tokyo)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>OKA, Satoru</au><au>SUZUKI, Hidenori</au><au>HASHIMOTO, Hideo</au><au>JYO, Toshihiko</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The Properties of Two Sea-Squirt Antigens</atitle><jtitle>Journal of biochemistry (Tokyo)</jtitle><addtitle>J Biochem</addtitle><date>1982-01</date><risdate>1982</risdate><volume>91</volume><issue>1</issue><spage>247</spage><epage>255</epage><pages>247-255</pages><issn>0021-924X</issn><eissn>1756-2651</eissn><abstract>The accuracy of radioimmunoassay (RIA) has been much improved for sea-squirt antigen Gi-x, which is considered to be of higher molecular weight than antigen Ei-M, by using radiolabeled Gi-x as a tracer. Gel chromatography monitored by the improved RIA revealed a wide distribution of molecular weight of Gi-x type antigens, in contrast to Ei-M. However, since a considerable portion of the anti-genic activity gave a single peak in gel chromatography with Sepharose 6B, the substance in the peak fractions was isolated as a fairly homogeneous preparation and referred to as Gi-rep. Gi-rep showed distinct characteristics of Gi-x type antigens and was clearly discriminated from Ei-M by radioimmunometry in vitro. The in vitro observation also suggested that Gi-rep and Ei-M carried a common antigenic determinant (type α), but that Ei-M also carried a specific determinant (type β). The weight-average molecular weights were 1.1×105 for Gi-rep and 2.3×104 for Ei-M. 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| ispartof | Journal of biochemistry (Tokyo), 1982-01, Vol.91 (1), p.247-255 |
| issn | 0021-924X 1756-2651 |
| language | eng |
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| source | J-STAGE Free; MEDLINE; Oxford University Press Journals Digital Archive Legacy; Free Full-Text Journals in Chemistry |
| subjects | Animals Antigens - analysis Antigens - immunology Antigens - isolation & purification Body Fluids - immunology Chromatography, Gel Epitopes - immunology Glycoproteins - analysis Molecular Weight Radioimmunoassay Urochordata - immunology |
| title | The Properties of Two Sea-Squirt Antigens |
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