The Properties of Two Sea-Squirt Antigens

The accuracy of radioimmunoassay (RIA) has been much improved for sea-squirt antigen Gi-x, which is considered to be of higher molecular weight than antigen Ei-M, by using radiolabeled Gi-x as a tracer. Gel chromatography monitored by the improved RIA revealed a wide distribution of molecular weight...

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Veröffentlicht in:Journal of biochemistry (Tokyo) 1982-01, Vol.91 (1), p.247-255
Hauptverfasser: OKA, Satoru, SUZUKI, Hidenori, HASHIMOTO, Hideo, JYO, Toshihiko
Format: Artikel
Sprache:eng
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Zusammenfassung:The accuracy of radioimmunoassay (RIA) has been much improved for sea-squirt antigen Gi-x, which is considered to be of higher molecular weight than antigen Ei-M, by using radiolabeled Gi-x as a tracer. Gel chromatography monitored by the improved RIA revealed a wide distribution of molecular weight of Gi-x type antigens, in contrast to Ei-M. However, since a considerable portion of the anti-genic activity gave a single peak in gel chromatography with Sepharose 6B, the substance in the peak fractions was isolated as a fairly homogeneous preparation and referred to as Gi-rep. Gi-rep showed distinct characteristics of Gi-x type antigens and was clearly discriminated from Ei-M by radioimmunometry in vitro. The in vitro observation also suggested that Gi-rep and Ei-M carried a common antigenic determinant (type α), but that Ei-M also carried a specific determinant (type β). The weight-average molecular weights were 1.1×105 for Gi-rep and 2.3×104 for Ei-M. Both preparations consisted of acidic glycoproteins with considerable amounts of sulfate and phosphate.
ISSN:0021-924X
1756-2651
DOI:10.1093/oxfordjournals.jbchem.a133681