Characterization of Normal Human Embryo Cells Grown to over 100 Population Doublings

Characterization of Normal Human Embryo Cells Grown to over 100 Population Doublings

Normal human embryonic cells were subcultured for over 100 population doublings without modification of the basic medium. The cells were evaluated for growth rate, confluent density, chromosome stability, growth in soft agar, ability to hydrolyze casein and tumorigenicity. The cells possessed the ch...

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Veröffentlicht in:In Vitro 1978-05, Vol.14 (5), p.405-412
Hauptverfasser: Poiley, J. A., R. F. Schuman, R. J. Pienta
Format: Artikel
Sprache:eng
Schlagworte:
Animal cells
Animals
Blood
Caseins - metabolism
Cell culture techniques
Cell Division
Cell growth
Cell Line
Cell lines
Cricetinae
Culture Media
Cultured cells
Diploidy
Embryo, Mammalian
Embryonic cells
Epithelial cells
Fibroblasts
Human growth
Humans
Karyotyping
Neoplasms, Experimental - etiology
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Zusammenfassung:Normal human embryonic cells were subcultured for over 100 population doublings without modification of the basic medium. The cells were evaluated for growth rate, confluent density, chromosome stability, growth in soft agar, ability to hydrolyze casein and tumorigenicity. The cells possessed the characteristics of normal cells. The batch of serum used to supplement the medium was found to be of primary importance in the long-term growth of this cell culture.
ISSN:0073-5655
1475-2689
DOI:10.1007/BF02616101