Separation of catheptic enzymes of bovine spleen by isoelectric focusing

Catheptic proteases, which are potentially useful for meat processing, have been resolved and identified in bovine spleen extract by free solution zone convection isoelectric focusing. The isozymes of cathepsins A,B,D,L, and H have been separated, and their isoelectric points have been determined. The method is a very convenient and useful technique for analytical and preparative applications. Advantages over other methods of isoelectric focusing include simplicity of operation, ease of recovery and analysis of separated components, and no interference from proteins precipitating at their isoelectric points.