Rapid hydrolysis of deoxynucleoside triphosphates accompanies DNA synthesis by T4 DNA polymerase and T4 accessory proteins 44/62 and 45

Bacteriophage T4 gene 44/62 and 45 accessory proteins stimulate DNA synthesis by T4 DNA polymerase. Using a o/X174 DNA template primed with a strand of a restriction fragment, these accessory proteins also greatly increase the hydrolysis of dNTP to dNMP which accompanies DNA synthesis by the polymerase. The rate of formation of a deoxynucleoside monophosphate is up to 10 times the rate of its incorporation into DNA. This rapid hydrolysis depends on the combined presence of the polymerase, the accessory proteins, and the template-primer. With a o/X174 template-primer, dCTP is hydrolyzed more efficiently than the other triphosphates; with activated salmon sperm DNA, dATP and dGTP are the most efficiently hydrolyzed. A likely explanation for the deoxynucleoside triphosphate hydrolysis is that the polymerase, when detained at hair-pin barriers in the template, will alternately incorporate and then, using its 3' exonuclease activity, remove deoxynucleoside monophosphates.