Isolation of pure, active α 2 macroglobulin from small-scale plasma samples

Physiologically “active” α 2 macroglobulin (α 2M) of at least 98% purity can be isolated in high yield from small-scale plasma samples regardless of plasma haptoglobin type. The technique described herein, involving cibacron blue sepharose chromatography and immunoadsorption is suitable for purifica...

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Veröffentlicht in:	Clinica chimica acta 1982-01, Vol.118 (1), p.21-31
Hauptverfasser:	Bridges, Michael A., Applegarth, Derek A., Johannson, Joanne, Davidson, A.George F., Wong, Lawrence T.K.
Format:	Artikel
Sprache:	eng
Schlagworte:	Adolescent alpha-Macroglobulins - isolation & purification Child Chromatography, Affinity - methods Electrophoresis, Polyacrylamide Gel Humans Macromolecular Substances Molecular Weight Peptide Hydrolases - metabolism Ultrafiltration
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Beschreibung
Zusammenfassung:	Physiologically “active” α 2 macroglobulin (α 2M) of at least 98% purity can be isolated in high yield from small-scale plasma samples regardless of plasma haptoglobin type. The technique described herein, involving cibacron blue sepharose chromatography and immunoadsorption is suitable for purification of α 2M from plasma samples of 5 ml.
ISSN:	0009-8981 1873-3492
DOI:	10.1016/0009-8981(82)90223-6