A New Subculture and Nematocidal Assay Using a Species of Diplogastridae

A New Subculture and Nematocidal Assay Using a Species of Diplogastridae

A new subculture method and a novel microplate assay for nematocidal activity using a species of Diplogastridae have been developed. The assay gives results rapidly, with high sensitivity in 4h, and indicated good correlation between action mechanism and the nematode shape when examining 15 known co...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Chemical & pharmaceutical bulletin 1998/08/15, Vol.46(8), pp.1261-1264
Hauptverfasser: YANAGIDA, Jun, MATSUHASHI, Rinako, WATANABE, Ie, SATOU, Tadaaki, KOIKE, Kazuo, NIKAIDO, Tamotsu, AKAO, Shinkichi
Format: Artikel
Sprache:eng
Schlagworte:
Animals
Antibiotics. Antiinfectious agents. Antiparasitic agents
Antinematodal Agents - pharmacology
Antiparasitic agents
avermectin
Biological and medical sciences
Culture Media
Diplogastridae
Drug Evaluation, Preclinical - methods
Medical sciences
microplate assay
nematocidal activity
Nematoda - drug effects
Nematoda - growth & development
nematode
Pharmacology. Drug treatments
subculture
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:A new subculture method and a novel microplate assay for nematocidal activity using a species of Diplogastridae have been developed. The assay gives results rapidly, with high sensitivity in 4h, and indicated good correlation between action mechanism and the nematode shape when examining 15 known compounds, including the antiparasitic avermectin, antimalarial quinine, and the γ-amino-n-butyric acidA (GABAA) activated Cl- channel antagonist picrotoxinin. Thus new assay could be used as a primary screening method for new nematocidal compounds.
ISSN:0009-2363
1347-5223
DOI:10.1248/cpb.46.1261