A procedure for the simultaneous determination of small quantities of hyaluronate and isomeric chondroitin sulfates by chondroitinases

A procedure for the simultaneous determination of small quantities of hyaluronate and isomeric chondroitin sulfates by chondroitinases

A procedure for the simultaneous determination of hyaluronate and isomeric chondroitin sulfates by chondroitinases AC II (EC 4.2.99.1) plus ABC (EC 4.2.2.4) is described. It is sensitive (detection limit 0.02 μmol disaccharide), reproducible (coefficient of variation within series ≤10%), and, when c...

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Veröffentlicht in:Analytical biochemistry 1981-11, Vol.118 (1), p.185-190
Hauptverfasser: Kleine, Tilmann O., Merten, Bernd
Format: Artikel
Sprache:eng
Schlagworte:
Animals
Chemical Phenomena
Chemistry
Chondroitin - analogs & derivatives
Chondroitin Sulfates - analysis
Chondroitinases and Chondroitin Lyases
Hyaluronic Acid - analysis
Hyaluronoglucosaminidase
Isomerism
Leeches - enzymology
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creator Kleine, Tilmann O.
Merten, Bernd
description A procedure for the simultaneous determination of hyaluronate and isomeric chondroitin sulfates by chondroitinases AC II (EC 4.2.99.1) plus ABC (EC 4.2.2.4) is described. It is sensitive (detection limit 0.02 μmol disaccharide), reproducible (coefficient of variation within series ≤10%), and, when compared with the leech hyaluronidase (EC 3.2.1.36) assay, proves to be accurate.
doi_str_mv 10.1016/0003-2697(81)90176-7
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subjects Animals
Chemical Phenomena
Chemistry
Chondroitin - analogs & derivatives
Chondroitin Sulfates - analysis
Chondroitinases and Chondroitin Lyases
Hyaluronic Acid - analysis
Hyaluronoglucosaminidase
Isomerism
Leeches - enzymology
title A procedure for the simultaneous determination of small quantities of hyaluronate and isomeric chondroitin sulfates by chondroitinases
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