Reversed-phase high-performance liquid chromatography of histones

Calf thymus histones are separated into the five classical components H1, H2A, H2B, H3, and H4 using reversed-phase high-performance liquid chromatography. This method is fast and sensitive; a single run takes 80 min and protein quantities ranging from 3 μg up to 1 mg can be separated. The primary s...

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Veröffentlicht in:	Analytical biochemistry 1981-11, Vol.118 (1), p.147-154
Hauptverfasser:	Certa, Ulrich, von Ehrenstein, Gunter
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Caenorhabditis - analysis Cattle Chemical Phenomena Chemistry Chromatography, High Pressure Liquid Histones - isolation & purification Saccharomyces cerevisiae - analysis Species Specificity Thymus Gland - analysis
Online-Zugang:	Volltext
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