Detection of Neisseria gonorrhoeae by ultraviolet illumination of NADH generated by an immobilized antibody-gonococcal enzyme complex

Frozen, cervical swabs were placed in a lysis buffer containing an immobilized antibody to the gonococcal enzyme, 1,2-propanediol oxidoreductase. The immobilized antibody—enzyme complex that formed was active after the addition of substrate (1,2-propanediol and NAD +) and this activity could be dete...

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Veröffentlicht in:	Immunology letters 1981-01, Vol.3 (6), p.377-380
Hauptverfasser:	McDonald, H.C., Simon, P.A., Takeguchi, M.M., Odstrchel, G., Weetall, H.H., Felman, Yehudi M.
Format:	Artikel
Sprache:	eng
Schlagworte:	Alcohol Oxidoreductases - isolation & purification Cervix Uteri - microbiology Female Fluorescent Antibody Technique Gonorrhea - diagnosis Gonorrhea - microbiology Humans NAD - immunology Neisseria gonorrhoeae Neisseria gonorrhoeae - enzymology Neisseria gonorrhoeae - isolation & purification
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Beschreibung
Zusammenfassung:	Frozen, cervical swabs were placed in a lysis buffer containing an immobilized antibody to the gonococcal enzyme, 1,2-propanediol oxidoreductase. The immobilized antibody—enzyme complex that formed was active after the addition of substrate (1,2-propanediol and NAD +) and this activity could be detected by visual inspection of NADH fluorescence under ultraviolet illumination.
ISSN:	0165-2478 1879-0542
DOI:	10.1016/0165-2478(81)90070-5