A paraffin-celloidin embedding method for studying soft-hard tissue interfaces
Formaldehyde-fixed tooth-gingiva specimens were embedded whole in low-viscosity nitrocellulose (celloidin) to maintain the soft-hard tissue relationships. The soft tissue-bearing portion was sliced off, demineralized in EDTA and re-infiltrated in paraffin wax. Sections were mounted using a special b...
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| Veröffentlicht in: | Archives of oral biology 1981, Vol.26 (9), p.753-755 |
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| container_end_page | 755 |
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| container_issue | 9 |
| container_start_page | 753 |
| container_title | Archives of oral biology |
| container_volume | 26 |
| creator | Linder, J.E. Longhurst, P. Johnson, N.W. |
| description | Formaldehyde-fixed tooth-gingiva specimens were embedded whole in low-viscosity nitrocellulose (celloidin) to maintain the soft-hard tissue relationships. The soft tissue-bearing portion was sliced off, demineralized in EDTA and re-infiltrated in paraffin wax. Sections were mounted using a special benzyl alcohol acetone-containing fluid to achieve complete flattening and successfully stained using a variety of stains. |
| doi_str_mv | 10.1016/0003-9969(81)90193-X |
| format | Article |
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The soft tissue-bearing portion was sliced off, demineralized in EDTA and re-infiltrated in paraffin wax. 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The soft tissue-bearing portion was sliced off, demineralized in EDTA and re-infiltrated in paraffin wax. Sections were mounted using a special benzyl alcohol acetone-containing fluid to achieve complete flattening and successfully stained using a variety of stains.</description><subject>Child, Preschool</subject><subject>Collodion</subject><subject>Dentistry</subject><subject>Gingiva - anatomy & histology</subject><subject>Histological Techniques</subject><subject>Humans</subject><subject>Paraffin</subject><subject>Tooth, Deciduous - anatomy & histology</subject><issn>0003-9969</issn><issn>1879-1506</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1981</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kLtOwzAUhi0EglJ4A5AyIRgMdpw48YJUVdykCpYO3SzHPgajJi62g9S3J6EVI9O5_ef2IXRByS0llN8RQhgWgovrmt4IQgXDqwM0oXUlMC0JP0STP8kJOo3xcwhLzukxOq4IEyUXE_Q6yzYqKGtdhzWs194Z12XQNmAG5z1rIX14k1kfsph6sx1z0duEP1QwWXIx9pC5LkGwSkM8Q0dWrSOc7-0ULR8flvNnvHh7epnPFlizskoYCgqF4YUhtGEqL4RiueZWgDICmjy3ljJbN7zWBeOEWE2Fym3FKlvYytZsiq52YzfBf_UQk2xdHM9XHfg-yorVoiRCDMJiJ9TBxxjAyk1wrQpbSYkcKcoRkRwRyZrKX4pyNbRd7uf3TQvmr2mPbajf7-ow_PjtIMioHXQajAugkzTe_b_gB7wBgn8</recordid><startdate>1981</startdate><enddate>1981</enddate><creator>Linder, J.E.</creator><creator>Longhurst, P.</creator><creator>Johnson, N.W.</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>1981</creationdate><title>A paraffin-celloidin embedding method for studying soft-hard tissue interfaces</title><author>Linder, J.E. ; Longhurst, P. ; Johnson, N.W.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c357t-e41e4d64d01b3a249a32c6f9ead9eb22ff13f8b68c43600fc19a2f737f4f7f83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1981</creationdate><topic>Child, Preschool</topic><topic>Collodion</topic><topic>Dentistry</topic><topic>Gingiva - anatomy & histology</topic><topic>Histological Techniques</topic><topic>Humans</topic><topic>Paraffin</topic><topic>Tooth, Deciduous - anatomy & histology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Linder, J.E.</creatorcontrib><creatorcontrib>Longhurst, P.</creatorcontrib><creatorcontrib>Johnson, N.W.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Archives of oral biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Linder, J.E.</au><au>Longhurst, P.</au><au>Johnson, N.W.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A paraffin-celloidin embedding method for studying soft-hard tissue interfaces</atitle><jtitle>Archives of oral biology</jtitle><addtitle>Arch Oral Biol</addtitle><date>1981</date><risdate>1981</risdate><volume>26</volume><issue>9</issue><spage>753</spage><epage>755</epage><pages>753-755</pages><issn>0003-9969</issn><eissn>1879-1506</eissn><abstract>Formaldehyde-fixed tooth-gingiva specimens were embedded whole in low-viscosity nitrocellulose (celloidin) to maintain the soft-hard tissue relationships. The soft tissue-bearing portion was sliced off, demineralized in EDTA and re-infiltrated in paraffin wax. Sections were mounted using a special benzyl alcohol acetone-containing fluid to achieve complete flattening and successfully stained using a variety of stains.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>7039569</pmid><doi>10.1016/0003-9969(81)90193-X</doi><tpages>3</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0003-9969 |
| ispartof | Archives of oral biology, 1981, Vol.26 (9), p.753-755 |
| issn | 0003-9969 1879-1506 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_73895099 |
| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | Child, Preschool Collodion Dentistry Gingiva - anatomy & histology Histological Techniques Humans Paraffin Tooth, Deciduous - anatomy & histology |
| title | A paraffin-celloidin embedding method for studying soft-hard tissue interfaces |
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