A paraffin-celloidin embedding method for studying soft-hard tissue interfaces

A paraffin-celloidin embedding method for studying soft-hard tissue interfaces

Formaldehyde-fixed tooth-gingiva specimens were embedded whole in low-viscosity nitrocellulose (celloidin) to maintain the soft-hard tissue relationships. The soft tissue-bearing portion was sliced off, demineralized in EDTA and re-infiltrated in paraffin wax. Sections were mounted using a special b...

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Veröffentlicht in:Archives of oral biology 1981, Vol.26 (9), p.753-755
Hauptverfasser: Linder, J.E., Longhurst, P., Johnson, N.W.
Format: Artikel
Sprache:eng
Schlagworte:
Child, Preschool
Collodion
Dentistry
Gingiva - anatomy & histology
Histological Techniques
Humans
Paraffin
Tooth, Deciduous - anatomy & histology
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Zusammenfassung:Formaldehyde-fixed tooth-gingiva specimens were embedded whole in low-viscosity nitrocellulose (celloidin) to maintain the soft-hard tissue relationships. The soft tissue-bearing portion was sliced off, demineralized in EDTA and re-infiltrated in paraffin wax. Sections were mounted using a special benzyl alcohol acetone-containing fluid to achieve complete flattening and successfully stained using a variety of stains.
ISSN:0003-9969
1879-1506
DOI:10.1016/0003-9969(81)90193-X