Liposome-DNA interaction: microcalorimetric study

The authors applied differential scanning calorimetry (DSC) for studying the thermodynamic characteristics of DNA-liposome interactions. At the first stage, the melting curves of the ‘order–disorder’ thermal transition for lipid component and of the ‘ helix-coil’ transition for DNA were obtained. At the second stage, the phase behavior of the DNA-lipid mixture as a function of both components (lipid/DNA ratio) was obtained. The liposome-DNA interaction was investigated comparing the melting curves of the pure components and the mixture.