IMP‐GMP 5′‐Nucleotidase from Rat Brain: Activation by Polyphosphates

: IMP‐GMP 5′‐nucleotidase has been purified to homogeneity from total rat brain extracts. This preparation showed a unique band (Mr 54,000 ± 1,509) in sodium dodecyl sulfate‐polyacrylamide gel electrophoresis. The enzyme presented the following properties: optimal pH value, 6.5–6.8; relative velocit...

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Veröffentlicht in:	Journal of neurochemistry 1998-09, Vol.71 (3), p.1241-1250
Hauptverfasser:	Marques, Agostinho F. P., Teixeira, Natércia A., Gambaretto, Claudio, Sillero, Antonio, Günther Sillero, Maria A.
Format:	Artikel
Sprache:	eng
Schlagworte:	5'-Nucleotidase - metabolism 5′‐Nucleotidase Analytical, structural and metabolic biochemistry Animals Artemia - metabolism Biological and medical sciences Brain - enzymology Brain cytosol Buffers Cations, Divalent - pharmacology Cations, Monovalent - pharmacology Chromatography Diadenosine tetraphosphate Dinucleoside polyphosphates Enzyme Activation - physiology Enzymes and enzyme inhibitors Fundamental and applied biological sciences. Psychology Guanosine Monophosphate - metabolism Hydrogen-Ion Concentration Hydrolases Inosine Monophosphate - metabolism Male Nucleotides - pharmacology Phosphotransferases - metabolism Polyphosphates - metabolism Polyphosphates - pharmacology Rats Rats, Sprague-Dawley Substrate Specificity
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Zusammenfassung:	: IMP‐GMP 5′‐nucleotidase has been purified to homogeneity from total rat brain extracts. This preparation showed a unique band (Mr 54,000 ± 1,509) in sodium dodecyl sulfate‐polyacrylamide gel electrophoresis. The enzyme presented the following properties: optimal pH value, 6.5–6.8; relative velocity measured in the presence of MgCl2, MnCl2, CoCl2, and NiCl2 (2 mM), 100, 60, 11, and
ISSN:	0022-3042 1471-4159
DOI:	10.1046/j.1471-4159.1998.71031241.x