Long-term infection of a cell culture from newborn mouse brain with the FNV strain of yellow fever virus (author's transl)

A cell culture from brains of one day old mice was infected with a high multiplicity of the French neurotropic strain of yellow fever virus (FNV); the infected cell culture produced and released infectious FNV for more than 180 days post-inoculation with titres between 10(0.6) and 10(6.4) PFU/ml. The cell sheet exhibited some rare plaques of round cells with a slow centrifugal extension; the destruction of the cell sheet was not complete before 200 days post-inoculation.