Immunocytochemical methods to study the distribution of Orientia tsutsugamushi in Leptotrombidium (Acari: Trombiculidae) chiggers

Immunocytochemical methods were developed and tested for their ability to detect the distribution of Orientia tsutsugamushi in paraffin sections of adult chiggers (Leptotrombidium imphalum Vercammen-Grandjean and Langston). Rickettsial antigen was detected by application of a simple direct or amplif...

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Veröffentlicht in:Journal of medical entomology 1998-07, Vol.35 (4), p.556-560
Hauptverfasser: Myint, K.S. (U.S. Army Medical Component, Bangkok, Thailand.), Linthicum, K.J, Tanskul, P, Lerdthusnee, K, Vaughn, D.W, Manomuth, C, Mongkolsirichaikul, D, Hansukjariya, P, Hastriter, M.W
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Sprache:eng
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Zusammenfassung:Immunocytochemical methods were developed and tested for their ability to detect the distribution of Orientia tsutsugamushi in paraffin sections of adult chiggers (Leptotrombidium imphalum Vercammen-Grandjean and Langston). Rickettsial antigen was detected by application of a simple direct or amplified immunocytochemistry procedure and an indirect immunofluorescent procedure. In the direct procedure alkaline phosphatase conjugation to the mouse polyclonal antibody to the Karp strain was followed by the HistoMark Red test system to detect rickettsial antigen. The amplification procedure used a similar method but used an unlabeled primary antibody followed by secondary biotinylated antimouse IgG, streptavidin-alkaline phosphatase, and the HistoMark Red test system. The immunofluorescent procedure included a biotinylated secondary antibody followed by addition of a streptavidin-FITC conjugate. Specific tissue tropisms in infected chiggers were observed in the salivary glands, nervous tissue, and ovaries of adult female mites in all procedures; however, nonspecific fluorescence of the chigger limited definitive identification of tissue tropisms with the indirect immunofluorescent procedure
ISSN:0022-2585
1938-2928
DOI:10.1093/jmedent/35.4.556