Antibody-dependent Enhancement of Plaque Formation on Cell Lines of Macrophage Origin -- A Sensitive Assay for Antiviral Antibody

Antibody-dependent Enhancement of Plaque Formation on Cell Lines of Macrophage Origin -- A Sensitive Assay for Antiviral Antibody

Sir William Dunn School of Pathology, South Parks Road, Oxford, OX1 3RE, U.K. An assay for antiviral antibody based on antibody-dependent plaque enhancement (ADPE) in the macrophage cell line P388D1 is described which is as sensitive as, or more sensitive than a radioimmune assay. The method is appl...

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Veröffentlicht in:Journal of general virology 1981-11, Vol.57 (1), p.119-125
Hauptverfasser: Peiris, J. S. M, Porterfield, J. S
Format: Artikel
Sprache:eng
Schlagworte:
Animals
Antibodies, Viral - analysis
Bunyaviridae - immunology
Cell Line
Herpesviridae - immunology
Macrophages - microbiology
Mengovirus - immunology
Methods
Radioimmunoassay
Togaviridae - immunology
Viral Plaque Assay
Virus Replication
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Zusammenfassung:Sir William Dunn School of Pathology, South Parks Road, Oxford, OX1 3RE, U.K. An assay for antiviral antibody based on antibody-dependent plaque enhancement (ADPE) in the macrophage cell line P388D1 is described which is as sensitive as, or more sensitive than a radioimmune assay. The method is applicable to a range of Togaviridae and Bunyaviridae although not to Picornaviridae and Herpesviridae. The variables affecting the assay are investigated. Keywords: Togaviridae, Bunyaviridae, antibodies, enhancement Received 6 May 1981; accepted 22 July 1981.
ISSN:0022-1317
1465-2099
DOI:10.1099/0022-1317-57-1-119