An immunofluorescent method for specific staining of eosinophil granule major basic protein

We have developed an indirect immunofluorescent method for detection of the guinea pig eosinophil granule major basic protein (MBP) in formalin-fixed, paraffinembedded tissues. The method is specific for MBP, permits MBP localization in cells and tissues, and eliminates eosinophil non-specific fluor...

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Veröffentlicht in:	Journal of immunological methods 1981-01, Vol.47 (2), p.227-238
Hauptverfasser:	Filley, Warren V., Ackerman, Steven J., Gleich, Gerald J.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Blood Proteins - immunology Cytoplasmic Granules - chemistry Eosinophil Granule Proteins Eosinophils - chemistry Fixatives - pharmacology Fluorescent Antibody Technique Goats Guinea Pigs Immune Sera - pharmacology Rabbits Ribonucleases
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Zusammenfassung:	We have developed an indirect immunofluorescent method for detection of the guinea pig eosinophil granule major basic protein (MBP) in formalin-fixed, paraffinembedded tissues. The method is specific for MBP, permits MBP localization in cells and tissues, and eliminates eosinophil non-specific fluorescent staining.
ISSN:	0022-1759 1872-7905
DOI:	10.1016/0022-1759(81)90123-X