Electron Microscopic Studies on Cell-Sized Bilayer Liposomes

GOTO, K. and TAMAHASHI, N. Electron Microscopic Studies on Cell-Sized Bilayer Liposomes. Tohoku J. exp. Med., 1981, 134 (1), 97-102 - Cell-sized bilayer liposomes have been visualized directly in the electron microscope by a thin section method. Fixation in lanthanum nitrate plus potassium permanganate has made this visualization possible. Trilamellar images were seen in thin sections of the fixed liposomes; measurements made from microdensitometer tracings revealed a thickness (peak-to-peak distance) ranging from 25.3 to 55.3Å. The mean peak-to-peak distance of 35.6±8.2 (±S.D.) was in good agreement with the value obtained by Robertson from typical myelin figures.