Rapid tubulin synthesis during ciliated cell differentiation

Rapid tubulin synthesis during ciliated cell differentiation

Using pulse-chase conditions in culture we have investigated the incorporation of 3H-leucine into tubulin of isolated oviducts from 5 day-old mice. Label appears in soluble, particulate and axonemal fractions minutes after incubation. In the latter two fractions, but not in the soluble fraction, thi...

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Veröffentlicht in:Cell biology international reports 1977-05, Vol.1 (3), p.275-281
Hauptverfasser: Dirksen, Ellen Roter, Staprans, Ilona
Format: Artikel
Sprache:eng
Schlagworte:
Animals
Cell Differentiation
Cilia - metabolism
Fallopian Tubes - cytology
Fallopian Tubes - metabolism
Female
Glycoproteins - biosynthesis
Kinetics
Mice
Morphogenesis
Tubulin - biosynthesis
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Zusammenfassung:Using pulse-chase conditions in culture we have investigated the incorporation of 3H-leucine into tubulin of isolated oviducts from 5 day-old mice. Label appears in soluble, particulate and axonemal fractions minutes after incubation. In the latter two fractions, but not in the soluble fraction, this label is rapidly diluted under chase conditions. The data do not fit a simple model of sequential transfer of radioactively labeled, newly synthesized tubulin from a soluble fraction through centriole precursors to assembled ciliary axonemes.
ISSN:0309-1651
1878-240X
DOI:10.1016/0309-1651(77)90053-4