ESTABLISHMENT OF A HUMAN RECTAL CANCER CELL LINE PRODUCING CARCINOEMBRYONIC ANTIGEN

A permanent cell line (S-7512) was established by cell culture from the subcutaneous metastatic tumor of a human rectal cancer and has been maintained for 18 months. Cultured cells grew as isolated and piled-up colonies of epithelial cells, each with a scanty cytoplasm and a large nucleus containing...

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Veröffentlicht in:GANN Japanese Journal of Cancer Research 1977/12/31, Vol.68(6), pp.775-780
Hauptverfasser: MACHIDA, Seiro, ISHIOKA, Takashi, TAKASHIMA, Kazutoshi, FUKUSHIMA, Matsuro, ISHIKAWA, Yoshinobu, KUDO, Hajime
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Sprache:eng
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Zusammenfassung:A permanent cell line (S-7512) was established by cell culture from the subcutaneous metastatic tumor of a human rectal cancer and has been maintained for 18 months. Cultured cells grew as isolated and piled-up colonies of epithelial cells, each with a scanty cytoplasm and a large nucleus containing several nucleoli. The average doubling time of this cell line was 57hr and plating efficiency was 30.1∼45.2% in a liquid medium. The modal chromosome number was 115 but a marker chromosome was not evident. Electron microscopic examination revealed many microvilli, free ribosomes, and abundant glycogen granules. Heterotransplantation of the cultured cells produced a large tumor and its microscopic examination showed the same histological appearance as the original tumor (carcinoma simplex). Carcinoembryonic antigen level of the spent medium of the confluent culture was 235.50ng/3.2×106 cells.
ISSN:0016-450X
DOI:10.20772/cancersci1959.68.6_775