Estrogen, Progesterone, and the Blood-Uterine Lumen Permeability Barrier in Rats
To investigate whether the existence of the permeability barrier between blood and the uterine lumen in rats is dependent upon hormone treatment, the effects of estradiol (E) and progesterone (P) on the abilities of [ 3 H] sucrose, [ 14 C] urea and tritiated water ([ 3 H] OH) to enter the uterine lu...
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Veröffentlicht in: | Biology of reproduction 1981-09, Vol.25 (2), p.314-320 |
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Zusammenfassung: | To investigate whether the existence of the permeability barrier between blood and the uterine
lumen in rats is dependent upon hormone treatment, the effects of estradiol (E) and progesterone
(P) on the abilities of [ 3 H] sucrose, [ 14 C] urea and tritiated water ([ 3 H] OH) to enter the uterine
lumen from blood were determined in immature, ovariectomized rats following i.v. injection of a
radiolabeled test substance. To facilitate radioactivity recovery from the uterine lumen, intrauterine saline injections were
administered to all animals except those treated continuously with E
only. In one series of experiments, animals were treated for 3 days with or without E (0.5 µg/day)
and P (2 mg/day) whereas, in another series, animals pretreated for 2 days with or without E
(0.5 µg/day) were treated on the following day with or without P (2 mg). In both series, animals
were killed 15 or 60 min after the i.v. injection. For [ 3 H] sucrose, the uterine fluid (UF) radioactivity concentrations did not change significantly with time and were significantly
lower than
those of serum at 15 min postinjection in animals receiving any given hormone treatment. When
[ 14 C] urea was administered to animals treated continuously with E, the radioactivity concentrations either did not differ significantly
between UF and serum or were significantly higher in UF.
In animals receiving any other hormone treatment, UF radioactivity concentrations were significantly less than those of serum
at 15 min postinjection, and whereas serum concentrations decreased with time, those of UF increased. By contrast, [ 3 H] OH entered the uterine lumen readily,
as indicated by radioactivity concentrations which were significantly higher in UF than in serum in
all groups. The results of the present experiments suggest that a blood-uterine lumen permeability
barrier to [ 3 H] sucrose exists independently of E and P and that the existence of a barrier to
[ 14 C] urea is hormone dependent. |
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ISSN: | 0006-3363 1529-7268 |
DOI: | 10.1095/biolreprod25.2.314 |