Chemical synthesis of two deoxyribododecanucleotides for the attachment of restriction termini to an artificial minigene

In order to permit in vivo cloning of an artificial minigene designed to code for a modified S-peptide, the phosphodiester method for the chemical synthesis of two dodecadeoxyribonucleotides is described. Each of the latter possesses antiparallel complementarity one of the wto minigene strands and t...

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Veröffentlicht in:	Gene 1977-07, Vol.1 (5), p.357-372
Hauptverfasser:	Poonian, M.S., McComas, W.W., Nussbaum, A.L.
Format:	Artikel
Sprache:	eng
Schlagworte:	Base Sequence cohesive ends Deoxyribonucleotides - chemical synthesis DNA, Recombinant EcoRI endonuclease Genetic Code Methods Oligodeoxyribonucleotides - chemical synthesis Oligonucleotides - chemical synthesis polynucleotide ligase Polynucleotide Ligases - metabolism RNA - genetics S-peptide from RNAase
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