Chemical synthesis of two deoxyribododecanucleotides for the attachment of restriction termini to an artificial minigene
In order to permit in vivo cloning of an artificial minigene designed to code for a modified S-peptide, the phosphodiester method for the chemical synthesis of two dodecadeoxyribonucleotides is described. Each of the latter possesses antiparallel complementarity one of the wto minigene strands and t...
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Veröffentlicht in: | Gene 1977-07, Vol.1 (5), p.357-372 |
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Hauptverfasser: | , , |
Format: | Artikel |
Sprache: | eng |
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Zusammenfassung: | In order to permit in vivo cloning of an artificial minigene designed to code for a modified S-peptide, the phosphodiester method for the chemical synthesis of two dodecadeoxyribonucleotides is described. Each of the latter possesses antiparallel complementarity one of the wto minigene strands and to the single-stranded
EcoRI-generated end. They can thus serve as cohesive termini (“splints”) for polynucleotide ligase joining. |
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ISSN: | 0378-1119 1879-0038 |
DOI: | 10.1016/0378-1119(77)90040-3 |