Differential labelling of α and β-noradrenergic receptors in calf cerebellum membranes with 3H-adrenaline

β-NORADRENERGIC receptor detection with radiolabelled catecholamines has been hampered by binding of catecholamines to other sites 1,2 . Lefkowitz and Williams 3 described binding of a synthetic catecholamine 3 H-hydroxybenzylisoprenaline to β-noradrenergic receptors on frog red blood cell membranes. Using procedures to prevent metabolism and nonspecific binding, we identified binding of 3 H-adrenaline to α-noradrenergic receptor sites in brain membranes 4,5 . We report here the labelling of β-noradrenergic receptors in membranes of the calf cerebellum with 3 H-adrenaline.