Crossed Immunoelectrophoretic Identification of Partially Purified Type Common and Type Specific Herpes Simplex Virus Glycoprotein Antigens

Summary HSV glycoprotein antigens obtained from Triton X-100-solubilized type 1- and type 2-infected cells were partially purified by ion-exchange chromatography and analyzed by crossed immunoelectropho-resis in Triton X-100 containing agarose gel. Two HSV type common, two HSV type 1 specific, and o...

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Veröffentlicht in:	Experimental biology and medicine (Maywood, N.J.) N.J.), 1977-11, Vol.156 (2), p.349-353
Hauptverfasser:	Vestergaard, Bent F., Grauballe, Per C.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Antibodies, Viral - biosynthesis Antigens, Viral - analysis Antigens, Viral - isolation & purification Epitopes Glycoproteins - immunology Immunoelectrophoresis, Two-Dimensional Rabbits Simplexvirus - immunology Viral Proteins - immunology
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Zusammenfassung:	Summary HSV glycoprotein antigens obtained from Triton X-100-solubilized type 1- and type 2-infected cells were partially purified by ion-exchange chromatography and analyzed by crossed immunoelectropho-resis in Triton X-100 containing agarose gel. Two HSV type common, two HSV type 1 specific, and one HSV type 2 specific antigens were identified. Rabbits immunized with the HSV glycoprotein preparations developed strong viral-neutralizing antibodies.
ISSN:	0037-9727 1535-3702 1535-3699
DOI:	10.3181/00379727-156-39934