A method of increasing EA positive cells in mouse spleen lymphocytes

A method of increasing EA positive cells in mouse spleen lymphocytes

Treatment of mouse spleen lymphocytes with a high concentration of trypsin (1 mg/ml) increases the number of EA positive cells to include nearly all the B lymphocytes. It seems that all B lymphocytes express Fc receptors but in some cells these are hidden by other proteins present on the cell surfac...

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Veröffentlicht in:Journal of immunological methods 1977-01, Vol.18 (3), p.317-320
Hauptverfasser: Papamichail, M., Pavlatou, M.
Format: Artikel
Sprache:eng
Schlagworte:
Animals
Lymphocytes - immunology
Mice
Mice, Inbred BALB C
Pronase - pharmacology
Rosette Formation - methods
Spleen - immunology
Thymus Gland - immunology
Time Factors
Trypsin - pharmacology
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Zusammenfassung:Treatment of mouse spleen lymphocytes with a high concentration of trypsin (1 mg/ml) increases the number of EA positive cells to include nearly all the B lymphocytes. It seems that all B lymphocytes express Fc receptors but in some cells these are hidden by other proteins present on the cell surfaces.
ISSN:0022-1759
1872-7905
DOI:10.1016/0022-1759(77)90185-5