Fluorescence quenching studies with proteins

Fluorescence quenching studies with proteins

A review is presented on the use of the technique of solute fluorescence quenching to study the structure and dynamics of proteins. A number of factors are discussed that must be considered in analyzing such data. Among these factors are the efficiency of the quenching process, the relative importan...

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Veröffentlicht in:Analytical Biochemistry 1981-07, Vol.114 (2), p.199-227
Hauptverfasser: Eftink, Maurice R., Ghiron, Camillo A.
Format: Artikel
Sprache:eng
Schlagworte:
Acetamides
Acrylamide
Acrylamides
Alcohol Oxidoreductases
Azurin
Chemical Phenomena
Chemistry, Physical
dynamics
Ethylene Chlorohydrin - analogs & derivatives
Fluorescence
Hydrogen Peroxide
Indoles
Kinetics
Liver - enzymology
Luminescent Measurements
Mathematics
Plant Proteins
Proteins
Pyridinium Compounds
reviews
Sodium Iodide
structure
Succinimides
Tryptophan
Tyrosine
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Zusammenfassung:A review is presented on the use of the technique of solute fluorescence quenching to study the structure and dynamics of proteins. A number of factors are discussed that must be considered in analyzing such data. Among these factors are the efficiency of the quenching process, the relative importance of static quenching, the heterogeneity of the emission, and the tendency of the quencher to interact with the protein.
ISSN:0003-2697
1096-0309
DOI:10.1016/0003-2697(81)90474-7