The incorporation of 5-fluoro-2′-deoxyuridine into DNA of mammalian tumor cells

The incorporation of 5-fluoro-2′-deoxyuridine into DNA of mammalian tumor cells

Treatment of L1210 mouse leukemia cells with 10 −6M [ 3h]5-fluoro-2′-deoxy-uridine resulted in the incorporation of radioactivity into DNA. The DNA hydrolysate was shown to contain [ 3h]5-fluoro-2′-deoxyuridylic acid by an unequivocal assay involving the formation of a tightly-bound complex with thy...

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Veröffentlicht in:Biochemical and biophysical research communications 1981-09, Vol.102 (2), p.654-658
Hauptverfasser: Danenberg, Peter V., Heidelberger, Charles, Mulkins, Mary A., Peterson, Andrew R.
Format: Artikel
Sprache:eng
Schlagworte:
Animals
DNA Replication
DNA, Neoplasm - biosynthesis
Floxuridine - metabolism
Kinetics
Leukemia L1210 - metabolism
Mice
Thymidylate Synthase - metabolism
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Zusammenfassung:Treatment of L1210 mouse leukemia cells with 10 −6M [ 3h]5-fluoro-2′-deoxy-uridine resulted in the incorporation of radioactivity into DNA. The DNA hydrolysate was shown to contain [ 3h]5-fluoro-2′-deoxyuridylic acid by an unequivocal assay involving the formation of a tightly-bound complex with thymidylate synthetase in the presence of 5,10-methylenetetrahydrofolate. The extent of incorporation was estimated to be 1 molecule of 5-fluoro-2′-deoxyuridylic acid per 10 DNA nucleotides.
ISSN:0006-291X
1090-2104
DOI:10.1016/S0006-291X(81)80182-9