Succinate Dehydrogenase-Coenzyme Q Reductase Assay in Leucocytes

Succinate Dehydrogenase-Coenzyme Q Reductase Assay in Leucocytes

The determination of the activity of succinate dehydrogenase-coenzyme Q reductase of leucocytes mitochondria has been studied by using 2, 6-dichlorophenolindophenol as an indicator. It was found that contaminating hemoglobin, disturbed the accurate estimation of the enzyme activity in the method pre...

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Veröffentlicht in:Chemical & pharmaceutical bulletin 1977/10/25, Vol.25(10), pp.2761-2762
Hauptverfasser: MASUGI, FUMINORI, SANMA, HIDEYUKI, NAKAMURA, TETSUYA
Format: Artikel
Sprache:eng
Schlagworte:
electron transport
Humans
Leukocytes - enzymology
Oxidoreductases - blood
Succinate Dehydrogenase - blood
Ubiquinone
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Zusammenfassung:The determination of the activity of succinate dehydrogenase-coenzyme Q reductase of leucocytes mitochondria has been studied by using 2, 6-dichlorophenolindophenol as an indicator. It was found that contaminating hemoglobin, disturbed the accurate estimation of the enzyme activity in the method previously reported. An improved assay method is presented.
ISSN:0009-2363
1347-5223
DOI:10.1248/cpb.25.2761