Purification of a phosphate carrier in pig heart mitochondria by affinity chromatography on mersalyl—ultrogel

This work describes the complete separation of the mersalyl and N-ethylmaleimide-sensitive protein from the nucleotide translocase. This purification was carried out by combining fractionation on a mersalyl-ultrogel column with purification on hydroxylapatite.

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Bibliographische Detailangaben
Veröffentlicht in:	FEBS letters 1981-06, Vol.128 (1), p.142-144
Hauptverfasser:	Touraille, Sylvie, Briand, Yves, Durand, Rogert, Bonnafous, Jean-Claude, Mani, Jean-Claude
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Chromatography, Affinity - methods EGTA Electrophoresis, Polyacrylamide Gel ethyleneglycol tetraacetic acid Ethylmaleimide - metabolism heart HEPES inorganic phosphate isolation mersalyl Mersalyl - metabolism mitochondria Mitochondria, Heart - analysis N-2-hydroxy-ethylpiperazine-N′-2, ethane sulfonic acid N-ethylmaleimide NEM Organomercury Compounds - metabolism Phosphates - metabolism pigs proteins Proteins - isolation & purification Sepharose Swine Tris Tris (hydroxymethyl)amino methane
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Beschreibung
Zusammenfassung:	This work describes the complete separation of the mersalyl and N-ethylmaleimide-sensitive protein from the nucleotide translocase. This purification was carried out by combining fractionation on a mersalyl-ultrogel column with purification on hydroxylapatite.
ISSN:	0014-5793 1873-3468
DOI:	10.1016/0014-5793(81)81100-3