Studies on Egg Drop Syndrome (EDS) and Chick Embryo Lethal Orphan (CELO) Avian Adenovirus DNAs by Restriction Endonucleases

Veterinary Research Institute of the Hungarian Academy of Sciences, H-1581, Budapest, Hungary Restriction endonucleases Eco RI, Bam HI, Hin dIII, Bgl I, Bgl II, Hpa I and Pst I recognized 48 cleavage sites in EDS adenovirus strain B8/78 DNA, whereas CELO virus DNA was cut into 61 fragments by the same enzymes. No similarity could be detected in the restriction patterns obtained from the two avian adenoviruses. The calculated mol. wt. of B8/78 DNA was 22.6 x 10 6 [about 34.2 kilobases (kb)] and that of CELO virus DNA was 28.9 x 10 6 (about 43.7 kb). The fragments generated from B8/78 DNA by Eco RI and Bam HI were physically mapped. Keywords: avian adenoviruses, DNA, restriction endonuclease analysis Received 26 February 1981; accepted 13 April 1981.