Three-dimensional computer reconstruction and display of neuronal structure

Shaded, three-dimensional images of microscopic neuronal structures are generated using a special computer graphics system known as THREAD ( Three-Dimensional Reconstruction Aand Display). This system uses both a computer and its associated image processing hard-ware and specialized computer software to produce both line-drawings (contour-grams) and shaded images obtained from 35 mm film negatives of cross-sectional images of microscopic neuronal structures. The resulting image is displayed on a TV monitor in either color or black and white and can be rotated to view all surfaces. Structural volume and planar surface areas are also evaluated during the procedure. Examples of a brainstem region and of a neuron nucleolus are presented.