Chemiluminescence during lipoxygenase-catalysed oxygenation of linoleic acid

Chemiluminescence during lipoxygenase-catalysed oxygenation of linoleic acid

Contrary to earlier observations (7) the present investigation shows that light emission from the lipoxygenase-catalysed oxygenation of linoleic acid can be readily measured in the absence of luminol with standard liquid scintillation counting equipment. The quenching effect of superoxide dismutase...

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Veröffentlicht in:Biochemical and biophysical research communications 1977-09, Vol.78 (1), p.424-428
Hauptverfasser: Veldink, Gerrit A., Jan Garssen, G., Slappendel, Steven, Vliegenthart, Johannes F.G., Boldingh, Jan
Format: Artikel
Sprache:eng
Schlagworte:
Kinetics
Linoleic Acids
Lipoxygenase - metabolism
Luminescent Measurements
Plants - enzymology
Superoxide Dismutase - metabolism
Superoxides
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Zusammenfassung:Contrary to earlier observations (7) the present investigation shows that light emission from the lipoxygenase-catalysed oxygenation of linoleic acid can be readily measured in the absence of luminol with standard liquid scintillation counting equipment. The quenching effect of superoxide dismutase suggests superoxide (O 2 • ) to play a key role in this process.
ISSN:0006-291X
1090-2104
DOI:10.1016/0006-291X(77)91271-2