Demonstration of two 3,3'-diaminobenzidine oxidation reactions associated with photosynthetic membranes in anaerobic light-grown Rhodospirillum rubrum

Demonstration of two 3,3'-diaminobenzidine oxidation reactions associated with photosynthetic membranes in anaerobic light-grown Rhodospirillum rubrum

Photosynthetic membranes of anaerobic light-grown Rhodospirillum rubrum oxidized 3,3'-diaminobenzidine. When glutaraldehyde-treated cells were exposed to 3,3'-diaminobenzidine in the light aerobically, the oxidation appeared to occur by two systems. One reaction was stimulated by white lig...

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Veröffentlicht in:The journal of histochemistry and cytochemistry 1977-11, Vol.25 (11), p.1264-1268
Hauptverfasser: Stevens, FJ, Pankratz, HS, Uffen, RL
Format: Artikel
Sprache:eng
Schlagworte:
3,3'-Diaminobenzidine
Bacteriorhodopsins - metabolism
Benzidines
Carotenoids - metabolism
Cyanides - pharmacology
Darkness
Light
Microscopy, Electron
Oxidation-Reduction
Rhodospirillum rubrum - analysis
Rhodospirillum rubrum - ultrastructure
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Zusammenfassung:Photosynthetic membranes of anaerobic light-grown Rhodospirillum rubrum oxidized 3,3'-diaminobenzidine. When glutaraldehyde-treated cells were exposed to 3,3'-diaminobenzidine in the light aerobically, the oxidation appeared to occur by two systems. One reaction was stimulated by white light and the second required molecular oxygen. The O2-dependent activity was inhibited by KCN.
ISSN:0022-1554
1551-5044
DOI:10.1177/25.11.410873