A gas chromatography-mass spectrometry method for the quantitative analysis of melatonin in plasma and cerebrospinal fluid

A method has been developed for the quantitative analysis of melatonin in human plasma and cerebrospinal fluid. The technique involves a simple one-step extraction with chloroform and conversion of the melatonin in the extract to its N-trimethylsilyl derivative, which is then measured by means of hi...

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Veröffentlicht in:Analytical biochemistry 1977-08, Vol.81 (2), p.283-291
Hauptverfasser: Wilson, Bary W., Snedden, Walter, Silman, R.E., Smith, Ivor, Mullen, Paul
Format: Artikel
Sprache:eng
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Zusammenfassung:A method has been developed for the quantitative analysis of melatonin in human plasma and cerebrospinal fluid. The technique involves a simple one-step extraction with chloroform and conversion of the melatonin in the extract to its N-trimethylsilyl derivative, which is then measured by means of high-sensitivity gas chromatography—mass spectrometry. Intra-assay precision for triplicate samples at the 20-pg/ml level was better than 20%, while the interassay precision for separate determinations made over the course of a week was better than 18%. In a series of human plasma samples taken over several times during a 24-hr period, a significant increase in melatonin level was noted during the hours of darkness.
ISSN:0003-2697
1096-0309
DOI:10.1016/0003-2697(77)90699-6